Blastocyst culture: Co-culture needs to be restored. Available culture media are still far from being optimal
نویسندگان
چکیده
منابع مشابه
Time to switch from co-culture to sequential defined media for transfer at the blastocyst stage.
It is current practice in in-vitro fertilization (IVF) to transfer embryos 48 h post-insemination at the 2–4 cell stage. However, it is now clear that this is a totally blind process. Firstly, embryos encounter an unsuitable environment, normally they would be in the oviduct. Secondly, motility of the uterus is related inversely to circulating progesterone. This problem of hypermotility of the ...
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Both cleavage-stage and blastocyst-stage embryo transfer policieshave advantages and drawbacks.Potential advantages of blastocyst culture and transfer in human IVF include: i. synchronization of the embryo with the female tract leading to increasedimplantation rates; thereby, reducing the need for multipleembryo transfers, ii. assessment of viability of an embryobefore transfer. This can be ach...
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It should now be beyond doubt that blastocyst culture results in better pregnancy rate (PR). This calls for fewer embryos to be transferred, and indeed blastocyst culture is of most value when the intention is to put back one embryo at a time. The PR with one top-grade blastocyst is 45-65% in different studies. If two top-grade blastocysts are transferred, the PR usually does not differ, but th...
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The use of sequential media has made extended culture and transfer of blastocysts feasible for human IVF. Embryo transfer on day 5 has been claimed to result in higher implantation rates than transfer on day 3, on the basis of retrospective comparative studies. This is not supported convincingly, however, in randomized controlled trials published to date. Blastocyst culture imposes additional r...
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ژورنال
عنوان ژورنال: Clinical Obstetrics, Gynecology and Reproductive Medicine
سال: 2018
ISSN: 2059-4828
DOI: 10.15761/cogrm.1000224